Tested Applications
| Positive WB detected in | Raji cells, mouse heart tissue |
| Positive IHC detected in | human heart tissue, mouse liver tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue, human rectal cancer tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10688-1-AP targets STRADB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1040 Product name: Recombinant human STRADB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 119-418 aa of BC008302 Sequence: ITTYWTVFTVGSWLWVISPFMAYGSASQLLRTYFPEGMSETLIRNILFGAVRGLNYLHQNGCIHRSIKASHILISGDGLVTLSGLSHLHSLVKHGQRHRAVYDFPQFSTSVQPWLSPELLRQDLHGYNVKSDIYSVGITACELASGQVPFQDMHRTQMLLQKLKGPPYSPLDISIFPQSESRMKNSQSGVDSGIGESVLVSSGTHTVNSDRLHTPSSKTFSPAFFSLVQLCLQQDPEKRPSASSLLSHVFFKQMKEESQDSILSLLPPAYNKPSISLPPVLPWTEPECDFPDEKDSYWEF Predict reactive species |
| Full Name | STE20-related kinase adaptor beta |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 28-31 kDa, 38-42 kDa |
| GenBank Accession Number | BC008302 |
| Gene Symbol | STRADB |
| Gene ID (NCBI) | 55437 |
| RRID | AB_2197715 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C0K7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The 418 amino acid STRADB protein, which is also named as ALS2CR2 and ILPIP, contains a putative N-terminal ATP-binding site, a protein kinase domain, and several C-terminal phosphorylation sites. STRADB potentiates the antiapoptotic activity of XIAP by enhancing XIAP-mediated activation of JNK1 and other JNK family members, but not by modulating XIAP-mediated caspase inhibition. This protein has 3 isoforms produced by alternative splicing with the MW of 47 kDa, 42 kDa and 31 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for STRADB antibody 10688-1-AP | Download protocol |
| WB protocol for STRADB antibody 10688-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product reports a 5-star experience with Western Blot on 293T and U2OS cells. The reviewer confirmed the target protein appeared at the expected molecular weight with no additional background bands in the relevant size range, describing the result as clean and reliable. Overall, the feedback highlights strong specificity and low background performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
anwesh (Verified Customer) (07-29-2019) | The protein was found at the right size. No additional background bands were observed around the size range. AWESOME!
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