STRADB Polyclonal antibody

STRADB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 10688-1-AP

Synonyms

ALS2CR2, CALS 21, ILP interacting protein, ILPIP, ILPIPA, PAPK, PRO1038, Pseudokinase ALS2CR2, STRAD beta, STRADB

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Validation Data Gallery

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  WB analysis of Raji using 10688-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10688-1-AP (STRADB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse heart using 10688-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10688-1-AP (STRADB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human heart using 10688-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 10688-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Raji cells, mouse heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10688-1-AP targets STRADB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STRADB fusion protein Ag1040
• Full Name: STE20-related kinase adaptor beta
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 28-31 kDa, 38-42 kDa
• GenBank Accession Number: BC008302
• Gene Symbol: STRADB
• Gene ID (NCBI): 55437
• RRID: AB_2197715
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 418 amino acid STRADB protein, which is also named as ALS2CR2 and ILPIP, contains a putative N-terminal ATP-binding site, a protein kinase domain, and several C-terminal phosphorylation sites. STRADB potentiates the antiapoptotic activity of XIAP by enhancing XIAP-mediated activation of JNK1 and other JNK family members, but not by modulating XIAP-mediated caspase inhibition. This protein has 3 isoforms produced by alternative splicing with the MW of 47 kDa, 42 kDa and 31 kDa.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size. No additional background bands were observed around the size range. AWESOME!

• Applications: Western Blot,
• Cell Tissue Type: 293T, U2OS

STRADB Antibody Western Blot, validation ( dilution) in 293T, U2OS (Cat no:10688-1-AP)