STRAP Monoclonal antibody

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• KD/KO Validated

STRAP Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

5E11F3

Cat no : 66712-1-Ig

Synonyms

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

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Validation Data Gallery

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  WB analysis of HEK-293 using 66712-1-Ig

  WB result of STRAP antibody (66712-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STRAP transfected HEK-293 cells.

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  WB analysis using 66712-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66712-1-Ig (STRAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 66712-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 66712-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 66712-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STRAP antibody (66712-1-Ig, Clone: 5E11F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66712-1-Ig targets STRAP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: STRAP fusion protein Ag13085
• Full Name: serine/threonine kinase receptor associated protein
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000162
• Gene Symbol: STRAP
• Gene ID (NCBI): 11171
• RRID: AB_2882063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.

Publications

Species	Application	Title
human	WB	Front Oncol; Cystatin-B Negatively Regulates the Malignant Characteristics of Oral Squamous Cell Carcinoma Possibly Via the Epithelium Proliferation/Differentiation Program; Authors - Tian-Tian Xu