STRAP Monoclonal antibody, PBS Only

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  WB analysis using 66712-1-Ig (same clone as 66712-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66712-1-Ig (STRAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

  WB result of STRAP antibody (66712-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STRAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STRAP antibody (66712-1-Ig, Clone: 5E11F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

STRAP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66712-1-PBS

Clone No.5E11F3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

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Product Information

66712-1-PBS targets STRAP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13085

  Product name: Recombinant human STRAP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC000162

  Sequence: MAMRQTPLTCSGHTRPVVDLAFSGITPYGYFLISACKDGKPMLRQGDTGDWIGTFLGHKGAVWGATLNKDATKAATAAADFTAKVWDAVSGDELMTLAHKHIVKTVDFTQDSNYLLTGGQDKLLRIYDLNKPEAEPKEISGHTSGIKKALWCSEDKQILSADDKTVRLWDHATMTEVKSLNFNMSVSSMEYIPEGEILVITYGRSIAFHSAVSLDPIKSFEAPATINSASLHPEKEFLVAGGEDFKLYKYDYNSGEELESYKGHFGPIHCVRFSPDGELYASGSEDGTLRLWQTVVGKTYGLWKCVLPEEDSGELAKPKIGFPETTEEELEEIASENSDCIFPSAPDVKA

• Full Name: serine/threonine kinase receptor associated protein
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000162
• Gene Symbol: STRAP
• Gene ID (NCBI): 11171
• RRID: AB_2882063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y3F4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.