Syntaxin 16 Polyclonal antibody

Syntaxin 16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11201-1-AP

Synonyms

Golgi Marker, hsyn16, STX16, SYN16, syntaxin 16

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Validation Data Gallery

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  WB analysis using 11201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11201-1-AP (Syntaxin 16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11201-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11201-1-AP (Syntaxin 16 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

11201-1-AP targets Syntaxin 16 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Syntaxin 16 fusion protein Ag1657
• Full Name: syntaxin 16
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC019042
• Gene Symbol: STX16
• Gene ID (NCBI): 8675
• RRID: AB_2198362
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin 16 (STX16), also named as SYN16, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes. STX16 is localized to the Golgi apparatus.

Publications

Species	Application	Title
mouse	WB	Hum Mol Genet; Interactome network analysis identifies multiple caspase-6 interactors involved in the pathogenesis of HD.; Authors - Sean-Patrick Riechers
human	IF	Eur J Immunol; SNARE protein expression and localization in human cytotoxic T lymphocytes.; Authors - Pattu Varsha V
human	WB	Traffic; Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.; Authors - Gustaf E Rydell
human	WB	J Virol; Potent Inhibition of HCMV by Modulating the Cellular SNARE Syntaxin 5.; Authors - Linda Cruz
human	WB	Biochem J; Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.; Authors - Nazma Malik