STX1A Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83159-6-RR (same clone as 83159-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83159-6-RR (STX1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 83159-6-RR (same clone as 83159-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using STX1A antibody (83159-6-RR, Clone: 230470F5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

STX1A Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83159-6-PBS

Clone No.230470F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1, HPC 1

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Product Information

83159-6-PBS targets STX1A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: syntaxin 1A (brain)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: NM_004603
• Gene Symbol: STX1A
• Gene ID (NCBI): 6804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q16623
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A (STX1A) and 1B (STX1B), two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody recognizes human STX1A.