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Syntaxin 6 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 10841-1-AP

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Con: 76 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Syntaxin 6 Antibody WB mouse brain tissue 10841-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP(Syntaxin 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

Syntaxin 6 Antibody IP mouse brain tissue 10841-1-AP

IP Result of anti-Syntaxin 6 (IP:10841-1-AP, 3ug; Detection:10841-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

Syntaxin 6 Antibody IF PC-12 cells 10841-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed PC-12 cells using 66477-1-Ig (TGN46 antibody) at dilution of 1:100 and 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66477-1-Ig, and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 10841-1-AP.

Syntaxin 6 Antibody IHC human prostate cancer tissue 10841-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP( Syntaxin 6 antibody at dilution of 1:50 (under 10x lens)

Syntaxin 6 Antibody IHC human prostate cancer tissue 10841-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP( Syntaxin 6 antibody at dilution of 1:50 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
PC-12 cells, murine BM-DC, murine Spleen-DC
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Syntaxin 6 fusion protein Ag1284
Full name:
syntaxin 6
Calculated molecular weight:
29 kDa
Observed molecular weight:
32 kDa
GenBank accession number:
BC009944
Gene ID (NCBI):
10228
Gene symbol
STX6
Synonyms
STX6, syntaxin 6

Background

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.


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