• Featured Product
  • KD/KO Validated

Syntaxin 6 Polyclonal antibody

Syntaxin 6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10841-1-AP

Synonyms

STX6, syntaxin 6



Tested Applications

Positive WB detected inNeuro-2a cells, human brain tissue, mouse brain tissue, rat brain tissue, PC-12 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells, murine BM-DC, murine Spleen-DC

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10841-1-AP targets Syntaxin 6 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntaxin 6 fusion protein Ag1284
Full Name syntaxin 6
Calculated molecular weight 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC009944
Gene symbol STX6
Gene ID (NCBI) 10228
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
IHC protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
IF protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
IP protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li

Protein Cell

HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus.

Authors - Wang Lifen L
humanWB

Biochem J

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.

Authors - Nazma Malik
mouseWB

Sci Rep

Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.

Authors - Xiaomin Su
  • KD Validated
mouseIF

J Immunol

Conventional dendritic cells require IRAP-Rab14 endosomes for efficient cross-presentation.

Authors - Weimershaus Mirjana M
mouseIF

Cell Rep

Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation.

Authors - Mirjana Weimershaus