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- KD/KO Validated
Syntaxin 6 Polyclonal antibody
Syntaxin 6 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 10841-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Neuro-2a cells, human brain tissue, mouse brain tissue, rat brain tissue, PC-12 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | PC-12 cells, murine BM-DC, murine Spleen-DC |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IF | See 7 publications below |
FC | See 1 publications below |
Product Information
10841-1-AP targets Syntaxin 6 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Syntaxin 6 fusion protein Ag1284 |
Full Name | syntaxin 6 |
Calculated molecular weight | 29 kDa |
Observed molecular weight | 32 kDa |
GenBank accession number | BC009944 |
Gene symbol | STX6 |
Gene ID (NCBI) | 10228 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
IHC protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
IF protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
IP protocol for Syntaxin 6 antibody 10841-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Protein Cell HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus. | ||
J Cell Biochem Thioxothiazolidin derivative, 4-OST, inhibits melanogenesis by enhancing the specific recruitment of tyrosinase-containing vesicles to lysosome. | ||
Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. | ||
Sci Rep Over-expression of microRNA-1 causes arrhythmia by disturbing intracellular trafficking system.
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