Syntaxin 6 Polyclonal antibody

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• Syntaxin 6 Polyclonal antibody (10841-1-AP)

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• KD/KO Validated

Syntaxin 6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10841-1-AP Copied!

Publications (29)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA
For FC, select conjugated version.

Synonyms

STX6, Syntaxin-6

• 

  WB analysis of A549 using 10841-1-AP

  WB result of Syntaxin 6 antibody (10841-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Syntaxin 6 transfected A549 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 10841-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody at dilution of 1:2000 incubated at 4 degree celsius over night.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 10841-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 10841-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 10841-1-AP

  IP result of anti-Syntaxin 6 (IP:10841-1-AP, 3ug; Detection:10841-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10841-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66477-1-Ig (TGN46 antibody) at dilution of 1:100 and 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:50, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 66477-1-Ig, and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 10841-1-AP.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of PC-12 using 10841-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of murine BM-DC using 10841-1-AP

  IF result of anti-STX6 (10841-1-AP) in Murine BM-DC by Dr. LOREDANA SAVEANU.

• 

  IF Staining of murine Spleen-DC using 10841-1-AP

  IF result of anti-STX6 (10841-1-AP) in Murine Spleen-DC by Dr. LOREDANA SAVEANU.

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Tested Applications

• Positive WB detected in: Neuro-2a cells, human brain tissue, mouse brain tissue, rat brain tissue, A549 cells, PC-12 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-12 cells, murine BM-DC, murine Spleen-DC

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:350-1:1400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10841-1-AP targets Syntaxin 6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1284

  Product name: Recombinant human STX6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-255 aa of BC009944

  Sequence: MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

• Full Name: syntaxin 6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC009944
• Gene Symbol: Syntaxin 6
• Gene ID (NCBI): 10228
• RRID: AB_2196506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43752
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Publications

What published studies show

The Syntaxin 6 Polyclonal antibody (10841-1-AP) has 29 citations across journals including Science, Cell, Nature Immunology, Nature Communications, Autophagy, Oncogene, J Virol, EMBO Journal, and Aging Cell. Research fields span intracellular trafficking, endosomal/Golgi biology, MHC class I cross-presentation, innate immunity, autophagy, viral infection (SARS-CoV-2, Lassa, bluetongue), cancer, atherosclerosis, and hepatotoxicity. Applications include WB, IF, and IHC in human, mouse, and rat systems.

Automatically generated from the 29 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Science; IRAP identifies an endosomal compartment required for MHC class I cross-presentation.; Authors - Loredana Saveanu
human	WB	Cell; The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins; Authors - Huaqing Deng
mouse	IF	Nat Immunol; IRAP(+) endosomes restrict TLR9 activation and signaling.; Authors - Joel Babdor
mouse		Autophagy; Lassa virus Z protein hijacks the autophagy machinery for efficient transportation by interrupting CCT2-mediated cytoskeleton network formation; Authors - Yueming Yuan
		Protein Cell; HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus.; Authors - Wang Lifen L
	IF	Nat Commun; IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses.; Authors - Irini Evnouchidou