Syntaxin 6 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84627-5-RR (same clone as 84627-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

• 

  WB analysis using 84627-5-RR (same clone as 84627-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 84627-5-RR (same clone as 84627-5-PBS)

  IP result of anti-Syntaxin 6 (IP:84627-5-RR, 4ug; Detection:84627-5-RR 1:1500) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84627-5-RR (same clone as 84627-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 84627-5-RR (same clone as 84627-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (84627-5-RR, Clone: 242100A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84627-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84627-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84627-5-RR against Human Syntaxin 6 were performed. The affinity constant is 1.55 nM.

Syntaxin 6 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 84627-5-PBS

Clone No.242100A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Syntaxin-6, STX6, 242100A9

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Product Information

84627-5-PBS targets Syntaxin 6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1284

  Product name: Recombinant human STX6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-255 aa of BC009944

  Sequence: MSMEDPFFVVKGEVQKAVNTAQGLFQRWTELLQDPSTATREEIDWTTNELRNNLRSIEWDLEDLDETISIVEANPRKFNLDATELSIRKAFITSTRQVVRDMKDQMSTSSVQALAERKNRQALLGDSGSQNWSTGTTDKYGRLDRELQRANSHFIEEQQAQQQLIVEQQDEQLELVSGSIGVLKNMSQRIGGELEEQAVMLEDFSHELESTQSRLDNVMKKLAKVSHMTSDRRQWCAIAILFAVLLVVLILFLVL

• Full Name: syntaxin 6
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC009944
• Gene Symbol: Syntaxin 6
• Gene ID (NCBI): 10228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O43752
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.