Filter: AllWBIHCIF-PFC (Intra)

WB analysis using 67137-1-Ig (same clone as 67137-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
WB analysis of Y79 using 67137-1-Ig (same clone as 67137-1-PBS)
Y79 cells were subjected to SDS PAGE followed by western blot with 67137-1-Ig (STXBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 67137-1-Ig (same clone as 67137-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 67137-1-Ig (same clone as 67137-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 67137-1-Ig (same clone as 67137-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67137-1-Ig (STXBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 67137-1-Ig (same clone as 67137-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STXBP1 antibody (67137-1-Ig, Clone: 1B5B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 67137-1-Ig (same clone as 67137-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human STXBP1 (67137-1-Ig, Clone:1B5B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.
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1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human STXBP1 (67137-1-Ig, Clone:1B5B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67137-1-PBS in a different storage buffer formulation.

STXBP1 Monoclonal antibody, PBS Only

STXBP1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Cat No. 67137-1-PBS

Clone No.1B5B3

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

1B5B3, EIEE4, FLJ37475, MUNC18 1, Munc18-1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67137-1-PBS targets STXBP1 in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28605 Product name: Recombinant human STXBP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 245-594 aa of BC015749 Sequence: HELTFQAMSYDLLPIENDVYKYETSGIGEARVKEVLLDEDDDLWIALRHKHIAEVSQEVTRSLKDFSSSKRMNTGEKTTMRDLSQMLKKMPQYQKELSKYSTHLHLAEDCMKHYQGTVDKLCRVEQDLAMGTDAEGEKIKDPMRAIVPILLDANVSTYDKIRIILLYIFLKNGITEENLNKLIQHAQIPPEDSEIITNMAHLGVPIVTDSTLRRRSKPERKERISEQTYQLSRWTPIIKDIMEDTIEDKLDTKHYPYISTRSSASFSTTAVSARYGHWHKNKAPGEYRSGPRLIIFILGGVSLNEMRCAYEVTQANGKWEVLIGSTHILTPQKLLDTLKKLNKTDEEISS Predict reactive species
Full Name	syntaxin binding protein 1
Calculated Molecular Weight	594 aa, 68 kDa
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC015749
Gene Symbol	STXBP1
Gene ID (NCBI)	6812
RRID	AB_2882436
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P61764
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

STXBP1, also named as UNC18A, N-Sec1 and p67 belongs to the STXBP/unc-18/SEC1 family. STXBP1 may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. It is essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. STXBP1 can interact with syntaxins 1, 2, and 3 but not syntaxin 4. It may play a role in determining the specificity of intracellular fusion reactions.