SUCLG1 Polyclonal antibody

SUCLG1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14923-1-AP

Synonyms

G ALPHA, SCS alpha, SUCLA1, SUCLG1



Tested Applications

Positive WB detected inHepG2 cells, rat liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inmouse kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14923-1-AP targets SUCLG1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUCLG1 fusion protein Ag6481
Full Name succinate-CoA ligase, alpha subunit
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC000504
Gene Symbol SUCLG1
Gene ID (NCBI) 8802
RRIDAB_2197177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).

Protocols

Product Specific Protocols
WB protocol for SUCLG1 antibody 14923-1-APDownload protocol
IHC protocol for SUCLG1 antibody 14923-1-APDownload protocol
IP protocol for SUCLG1 antibody 14923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Struct Mol Biol

TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.

Authors - Xinxin Zuo
humanWB

Mol Cell

Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation

Authors - Sophie Trefely
mouseWB

Nat Commun

A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages

Authors - Pengkai Sun
mouseWB,IP

J Mol Cell Cardiol

Mitochondrial protein hyperacetylation underpins heart failure with preserved ejection fraction in mice.

Authors - Xin Liu