SUCLG1 Recombinant monoclonal antibody

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  WB analysis using 86072-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86072-1-RR (SUCLG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86072-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUCLG1 antibody (86072-1-RR, Clone: 250424C8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SUCLG1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86072-1-RR

Clone No.250424C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EC:6.2.1.4, G ALPHA, SCS alpha, SCS-alpha, Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial

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Tested Applications

• Positive WB detected in: A549 cells, mouse kidney tissue, rat kidney tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86072-1-RR targets SUCLG1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6481

  Product name: Recombinant human SUCLG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-333 aa of BC000504

  Sequence: MVSGSSGLAAARLLSRSFLLPQNGIRHCSYTASRQHLYVDKNTKIICQGFTGKQGTFHSQQALEYGTKLVGGTTPGKGGQTHLGLPVFNTVKEAKEQTGATASVIYVPPPFAAAAINEAIEAEIPLVVCITEGIPQQDMVRVKHKLLRQEKTRLIGPNCPGVINPGECKIGIMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLCVGIGGDPFNGTDFIDCLEIFLNDSATEGIILIGEIGGNAEENAAEFLKQHNSGPNSKPVVSFIAGLTAPPGRRMGHAGAIIAGGKGGAKEKISALQSAGVVVSMSPAQLGTTIYKEFEKRKML

• Full Name: succinate-CoA ligase, alpha subunit
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC000504
• Gene Symbol: SUCLG1
• Gene ID (NCBI): 8802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P53597
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).