CoraLite® Plus 488-conjugated SUCLG1 Recombinant monoclonal antibody

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  IF Staining of A549 using CL488-86072

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 SUCLG1 antibody (CL488-86072, Clone: 250424C8 ) at dilution of 1:200.

SUCLG1 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-86072

Clone No.250424C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

EC:6.2.1.4, G ALPHA, SCS alpha, SCS-alpha, Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial

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Tested Applications

• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-86072 targets SUCLG1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6481

  Product name: Recombinant human SUCLG1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-333 aa of BC000504

  Sequence: MVSGSSGLAAARLLSRSFLLPQNGIRHCSYTASRQHLYVDKNTKIICQGFTGKQGTFHSQQALEYGTKLVGGTTPGKGGQTHLGLPVFNTVKEAKEQTGATASVIYVPPPFAAAAINEAIEAEIPLVVCITEGIPQQDMVRVKHKLLRQEKTRLIGPNCPGVINPGECKIGIMPGHIHKKGRIGIVSRSGTLTYEAVHQTTQVGLGQSLCVGIGGDPFNGTDFIDCLEIFLNDSATEGIILIGEIGGNAEENAAEFLKQHNSGPNSKPVVSFIAGLTAPPGRRMGHAGAIIAGGKGGAKEKISALQSAGVVVSMSPAQLGTTIYKEFEKRKML

• Full Name: succinate-CoA ligase, alpha subunit
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC000504
• Gene Symbol: SUCLG1
• Gene ID (NCBI): 8802
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P53597
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).