SULT1B1 Polyclonal antibody

SULT1B1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16050-1-AP

Synonyms

SULT1B2, ST1B2, ST1B1, EC:2.8.2.1

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Validation Data Gallery

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  WB analysis using 16050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 16050-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16050-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16050-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 16050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 16050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:240 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse colon tissue, human colon tissue, human liver tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:120-1:480

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16050-1-AP targets SULT1B1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SULT1B1 fusion protein Ag9009
• Full Name: sulfotransferase family, cytosolic, 1B, member 1
• Calculated Molecular Weight: 296 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC010895
• Gene Symbol: SULT1B1
• Gene ID (NCBI): 27284
• RRID: AB_2302767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).