SULT1B1 Recombinant monoclonal antibody

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  WB analysis of mouse colon using 83228-1-RR

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 83228-1-RR (SULT1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 83228-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 83228-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83228-1-RR (SULT1B1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A431 using 83228-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug SULT1B1 Recombinant antibody (83228-1-RR, Clone:240119C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 83228-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83228-1-RR against Human SULT1B1 were performed. The affinity constant is 63.8 nM.

SULT1B1 Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83228-1-RR

Clone No.240119C12

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

240119C12, EC:2.8.2.1, ST1B1, ST1B2, SULT1B2

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Tested Applications

• Positive WB detected in: mouse colon tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83228-1-RR targets SULT1B1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9009

  Product name: Recombinant human SULT1B1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-296 aa of BC010895

  Sequence: MLSPKDILRKDLKLVHGYPMTCAFASNWEKIEQFHSRPDDIVIATYPKSGTTWVSEIIDMILNDGDIEKCKRGFITEKVPMLEMTLPGLRTSGIEQLEKNPSPRIVKTHLPTDLLPKSFWENNCKMIYLARNAKDVSVSYYHFDLMNNLQPFPGTWEEYLEKFLTGKVAYGSWFTHVKNWWKRKEEHPILFLYYEDMKENPKEEIKKIIRFLEKNLNDEILDRIIHHTSFEVMKDNPLVNYTHLPTTVMDHSKSPFMRKGTAGDWKNYFTVAQNEKFDAIYETEMSKTALQFRTEI

• Full Name: sulfotransferase family, cytosolic, 1B, member 1
• Calculated Molecular Weight: 296 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC010895
• Gene Symbol: SULT1B1
• Gene ID (NCBI): 27284
• RRID: AB_3670911
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O43704
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (09-25-2025)

Very good antibody for WB. Strong signal and clean background

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse liver

SULT1B1 Antibody Western Blot validation (1:4000 dilution) in Mouse liver (Cat no:83228-1-RR)