SULT1C2 Polyclonal antibody

SULT1C2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10662-1-AP

Synonyms

humSULTC2, ST1C1, ST1C2, Sulfotransferase 1C1, Sulfotransferase 1C2, SULT1C#1, SULT1C1, SULT1C2

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Validation Data Gallery

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  WB analysis of human stomach using 10662-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 10662-1-AP (SULT1C2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 10662-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 10662-1-AP (SULT1C2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 10662-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10662-1-AP (SULT1C2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10662-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10662-1-AP (SULT1C2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10662-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10662-1-AP (SULT1C2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10662-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10662-1-AP (SULT1C2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10662-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10662-1-AP (SULT1C2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human stomach tissue, human kidney tissue, human liver tissue, mouse liver tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

10662-1-AP targets SULT1C2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SULT1C2 fusion protein Ag1064
• Full Name: sulfotransferase family, cytosolic, 1C, member 2
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC005353
• Gene Symbol: SULT1C2
• Gene ID (NCBI): 6819
• RRID: AB_2197745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT1C2, also named as SULT1C1, ST1C2, ST1C1, and humSULTC2, belongs to the sulfotransferase 1 family. It is a sulfotransferase enzymes that catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. SULT1C2 may be involved in the activation of carcinogenic hyroxylamines. It is responsible for transferring a sulfo moiety from PAPS to phenol-containing compounds.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; Correlation of Reactive Oxygen Species Levels with Resveratrol Sensitivities of Anaplastic Thyroid Cancer Cells.; Authors - Xu Zheng
human,rat	WB,IHC	Biochem Pharmacol; Identification of metabolic pattern and bioactive form of resveratrol in human medulloblastoma cells.; Authors - Shu Xiao-Hong XH
human	WB	FEBS J; Distinct sulfonation activities in resveratrol-sensitive and resveratrol-insensitive human glioblastoma cells.; Authors - Sun Zheng Z
human	WB,IHC	PLoS One; Metabolic patterns and biotransformation activities of resveratrol in human glioblastoma cells: relevance with therapeutic efficacies.; Authors - Shu Xiao-Hong XH
human	IHC,WB	J Cancer; Increased Reactive Oxygen Species and Distinct Oxidative Damage in Resveratrol-suppressed Glioblastoma Cells.; Authors - Bin Jia
rat	WB	Nutr Metab (Lond); Integrative proteomic and lipidomic analysis of Kaili Sour Soup-mediated attenuation of high-fat diet-induced nonalcoholic fatty liver disease in a rat model.; Authors - Shuo Cong