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SULT1E1 Polyclonal antibody, PBS Only (12522-1-PBS)

Filter: AllWBIPIHC

WB analysis of human lung using 12522-1-AP (same clone as 12522-1-PBS)
human lung tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.
WB analysis of human kidney using 12522-1-AP (same clone as 12522-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.
WB analysis of human liver using 12522-1-AP (same clone as 12522-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 12522-1-AP (same clone as 12522-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.
WB analysis of human adrenal gland using 12522-1-AP (same clone as 12522-1-PBS)
human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.
IP experiment of mouse liver using 12522-1-AP (same clone as 12522-1-PBS)
IP result of anti-SULT1E1 (IP:12522-1-AP, 4ug; Detection:12522-1-AP 1:400) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SULT1E1 Polyclonal antibody, PBS Only

Name: SULT1E1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 12522-1-PBS

SULT1E1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12522-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

12522-1-PBS targets SULT1E1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3216 Product name: Recombinant human SULT1E1 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-294 aa of BC027956 Sequence: MNSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPKSGTTWVSEIVYMIYKEGDVEKCKEDVIFNRIPFLECRKENLMNGVKQLDEMNSPRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYYFFLMVAGHPNPGSLPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKEDIRKEVIKLIHFLERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIMNQKLSPFMRKGITGDWKNHFTVALNEKFDKHYEQQMKESTLKFRTEI Predict reactive species
Full Name	sulfotransferase family 1E, estrogen-preferring, member 1
Calculated Molecular Weight	294 aa, 35 kDa
Observed Molecular Weight	32-35 kDa
GenBank Accession Number	BC027956
Gene Symbol	SULT1E1
Gene ID (NCBI)	6783
RRID	AB_2302772
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49888
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.