SUMF2 Polyclonal antibody

SUMF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11210-1-AP

Synonyms

Sulfatase-modifying factor 2, sulfatase modifying factor 2, PSEC0171, pFGE, Paralog of formylglycine-generating enzyme

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Validation Data Gallery

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  WB analysis using 11210-1-AP

  A431 cell lysates were subjected to SDS PAGE followed by western blot with 11210-1-AP (SUMF2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skin using 11210-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11210-1-AP (SUMF2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 11210-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11210-1-AP (SUMF2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 11210-1-AP

  IP result of anti-SUMF2 (IP:11210-1-AP, 4ug; Detection:11210-1-AP 1:2000) with A431 cells lysate 2640 ug.

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  IP experiment of HeLa using 11210-1-AP

  IP result of anti-SUMF2 (IP:11210-1-AP, 4ug; Detection:11210-1-AP 1:2000) with HeLa cells lysate 1560 ug.

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  IHC staining of human breast cancer using 11210-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11210-1-AP (SUMF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11210-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11210-1-AP (SUMF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 11210-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SUMF2 antibody (11210-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, human skin tissue, human spleen tissue
• Positive IP detected in: A431 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

11210-1-AP targets SUMF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUMF2 fusion protein Ag1711
• Full Name: sulfatase modifying factor 2
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC015600
• Gene Symbol: SUMF2
• Gene ID (NCBI): 25870
• RRID: AB_2255511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB,IHC	Int J Clin Exp Pathol; Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation.; Authors - Chuanfeng Fang
human	WB,IHC	Front Immunol; Advancing bladder cancer management: development of a prognostic model and personalized therapy; Authors - Xiang Huang