ChromoTek SUMO3-Trap Magnetic Agarose

Validation Data Gallery

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  The SUMO3-Trap Magnetic Agarose (s3tma) was used to immunoprecipitate endogenous SUMO3ylated and SUMO2ylated proteins from a human cell line. Samples of the input lysate (I), non-bound flow-through (F) and bound (B) fraction were analysed using Western blot. SUMO2/3ylated proteins are highly enriched using the SUMO3-Trap. Data kindly provided by Prof. Alfred Vertegaal, Leiden University, The Netherlands.

The ChromoTek SUMO3-Trap Agarose consists of an anti-SUMO3 NANOBODY®/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of SUMO3 and SUMO2 proteins from cell extracts of various organisms.

Cat No. s3tma

Specificity

SUMO2 and SUMO3

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Agarose

Synonyms

SUMO3, SUMO2, SMT3H1, SMT3A, SMT3 homolog 1

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Product Information

The ChromoTek SUMO3-Trap Agarose consists of an anti-SUMO3 NANOBODY®/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of SUMO3 and SUMO2 proteins from cell extracts of various organisms.

• Description: Immunoprecipitation of endogenous SUMO2 and SUMO3 proteins with anti-SUMO3 Nanobody conjugated to magnetic agarose beads.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to human SUMO2 and SUMO3 (Small Ubiquitin-like Modifier 2 and 3). The ChromoTek SUMO3-Trap Agarose will immunoprecipitate both monovalent SUMO2/3 and chains formed by the process of SUMOylation. In addition to human SUMO2 und SUMO3, it will also bind to homologs from other mammals.
• Binding Capacity: 25 ug of SUMO3 protein (~40 kDa) per 25 ul bead slurry
• Conjugate: Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
• Wash Buffer Compatibility: 0.5 M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 1 M Urea
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): /
• Compatibility with mass spectrometry: The SUMO3-Trap is optimized for on-bead digestion. For the application note, please click here: ; On-bead digest protocol for mass spectrometry
• RRID: AB_3716418
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze!

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