CoraLite® Plus 488-conjugated SUN2 Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-85103-3

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SUN2 antibody (CL488-85103-3, Clone: 242533G3 ) at dilution of 1:600.

SUN2 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85103-3

Clone No.242533G3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

UNC84B, 242533G3, FRIGG, KIAA0668, Protein unc-84 homolog B

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85103-3 targets SUN2 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16077

  Product name: Recombinant human UNC84B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 234-551 aa of BC111717

  Sequence: YPYGLQTFHPALVSWWAAKDSRRPDEGWEARDSSPHFQAEQRVMSRVHSLERRLEALAAEFSSNWQKEAMRLERLELRQGAPGQGGGGGLSHEDTLALLEGLVSRREAALKEDFRRETAARIQEELSALRAEHQQDSEDLFKKIVRASQESEARIQQLKSEWQSMTQESFQESSVKELRRLEDQLAGLQQELAALALKQSSVAEEVGLLPQQIQAVRDDVESQFPAWISQFLARGGGGRVGLLQREEMQAQLRELESKILTHVAEMQGKSAREAAASLSLTLQKEGVIGVTEEQVHHIVKQALQRYSEDRIGLADYAL

• Full Name: unc-84 homolog B (C. elegans)
• Calculated Molecular Weight: 717 aa, 80 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC111717
• Gene Symbol: SUN2
• Gene ID (NCBI): 25777
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UH99
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).