SUPV3L1 Polyclonal antibody

SUPV3L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12826-1-AP

Synonyms

SUV3-like protein 1, SUV3 like protein 1, SUV3, Suppressor of var1 3-like protein 1, EC:3.6.4.13

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Validation Data Gallery

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  WB analysis using 12826-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 12826-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12826-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12826-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 12826-1-AP

  HeLa cell were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:500.

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  IP experiment of HeLa using 12826-1-AP

  IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 12826-1-AP

  IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 12826-1-AP

  IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.

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  IHC staining of human stomach cancer using 12826-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12826-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12826-1-AP targets SUPV3L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SUPV3L1 fusion protein Ag3542
• Full Name: suppressor of var1, 3-like 1 (S. cerevisiae)
• Calculated Molecular Weight: 786 aa, 88 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC036112
• Gene Symbol: SUPV3L1
• Gene ID (NCBI): 6832
• RRID: AB_2877885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).

Publications

Species	Application	Title
human	WB	J Inherit Metab Dis; Mitochondrial RNA processing defect caused by a SUPV3L1 mutation in two siblings with a novel neurodegenerative syndrome.; Authors - Selma L van Esveld
human	WB	Biochimie; Cathepsin D inhibition during neuronal differentiation selectively affects individual proteins instead of overall protein turnover; Authors - Johannes Schneider