SURF1 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• SURF1 Polyclonal antibody, PBS Only (15379-1-PBS)

• 

  WB analysis using 15379-1-AP (same clone as 15379-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15379-1-AP (SURF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

  IP result of anti-SURF1 (IP:15379-1-AP, 4ug; Detection:15379-1-AP 1:2000) with HeLa cells lysate 1575 ug. This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 15379-1-AP (same clone as 15379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15379-1-AP (SURF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15379-1-AP (same clone as 15379-1-PBS)

  Immunofluorescent analysis of HeLa cells using 15379-1-AP (SURF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15379-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SURF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 15379-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

SURF 1, SURF-1, surfeit 1, Surfeit locus protein 1

---

Product Information

15379-1-PBS targets SURF1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3959

  Product name: Recombinant human SURF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC028314

  Sequence: MAAVAALQLGLRAAGLGRAPASAAWRSVLRVSPRPGVAWRPSRCGSSAAEASATKAEDDSFLQWVLLLIPVTAFGLGTWQVQRRKWKLNLIAELESRVLAEPVPLPADPMELKNLEYRPVKVRGCFDHSKELYMMPRTMVDPVREAREGGLISSSTQSGAYVVTPFHCTDLGVTILVNRGFVPRKKVNPETRQKGQIEGEVDLIGMVRLTETRQPFVPENNPERNHWHYRDLEAMARITGAEPIFIDANFQSTVPGGPIGGQTRVTLRNEHLQYIVTWYGLSAATSYLWFKKFLRGTPGV

• Full Name: surfeit 1
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-31 kDa
• GenBank Accession Number: BC028314
• Gene Symbol: SURF1
• Gene ID (NCBI): 6834
• RRID: AB_2239968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15526
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.