SUV39H1 Polyclonal antibody, PBS Only

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• SUV39H1 Polyclonal antibody, PBS Only (10574-1-PBS)

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• KD/KO Validated

SUV39H1 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 10574-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

• 

  WB analysis using 10574-1-AP (same clone as 10574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10574-1-AP (same clone as 10574-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse small intestine using 10574-1-AP (same clone as 10574-1-PBS)

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10574-1-AP (same clone as 10574-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10574-1-AP (same clone as 10574-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 10574-1-AP (same clone as 10574-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 10574-1-AP (same clone as 10574-1-PBS)

  IP result of anti-SUV39H1 (IP:10574-1-AP, 4ug; Detection:10574-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

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Product Information

10574-1-PBS targets SUV39H1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0854

  Product name: Recombinant human SUV39H1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 7-277 aa of BC006238

  Sequence: GCSVCCKSSWNQLQDLCRLAKLSCPALGISKRNLYDFEVEYLCDYKKIREQEYYLVKWRGYPDSESTWEPRQNLKCVRILKQFHKDLERELLRRHHRSKTPRHLDPSLANYLVQKAKQRRALRRWEQELNAKRSHLGRITVENEVDLDGPPRAFVYINEYRVGEGITLNQVAVGCECQDCLWAPTGGCCPGASLHKFAYNDQGQVRLRAGLPIYECNSRCRCGYDCPNRVVQKGIRYDLCIFRTDDGRGWGVRTLEKIRKNSFVMEYVGEI

• Full Name: suppressor of variegation 3-9 homolog 1 (Drosophila)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC006238
• Gene Symbol: SUV39H1
• Gene ID (NCBI): 6839
• RRID: AB_2196710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43463
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.