CoraLite® Plus 488-conjugated SUZ12 Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-84895-3

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SUZ12 antibody (CL488-84895-3, Clone: 242491B3 ) at dilution of 1:200, CL594-Phalloidin (red).

SUZ12 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84895-3

Clone No.242491B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

ChET 9 protein, CHET9, JJAZ1, Joined to JAZF1 protein, KIAA0160

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84895-3 targets SUZ12 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: suppressor of zeste 12 homolog (Drosophila)
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: NM_015355
• Gene Symbol: SUZ12
• Gene ID (NCBI): 23512
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15022
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.