SV2C Recombinant monoclonal antibody

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• SV2C Recombinant monoclonal antibody (88136-1-RR)

SV2C Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 88136-1-RR

Clone No.260377A4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Synaptic vesicle glycoprotein 2C

• 

  WB analysis using 88136-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88136-1-RR (SV2C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 88136-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88136-1-RR (SV2C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C6 cells, mouse cerebellum tissue, mouse brain tissue, rat brain tissue, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88136-1-RR targets SV2C in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg9076

  Product name: Recombinant human SV2C protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 459-578 aa of NM_014979.4

  Sequence: KPLQSDEYALLTRNVERDKYANFTINFTMENQIHTGMEYDNGRFIGVKFKSVTFKDSVFKSCTFEDVTSVNTYFKNCTFIDTVFDNTDFEPYKFIDSEFKNCSFFHNKTGCQITFDDDYS

• Full Name: synaptic vesicle glycoprotein 2C
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_014979.4
• Gene Symbol: SV2C
• Gene ID (NCBI): 22987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q496J9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Members of the synaptic vesicle glycoprotein 2 (SV2) family of proteins are involved in synaptic function throughout the brain. The ubiquitously expressed SV2A has been widely implicated in epilepsy, SV2C as a mediator of dopamine homeostasis and report that disrupted expression of SV2C within the basal ganglia is a pathological feature of PD. Genetic deletion of SV2C leads to reduced dopamine release in the dorsal striatum as measured by fast-scan cyclic voltammetry, reduced striatal dopamine content, disrupted α-synuclein expression, deficits in motor function, and alterations in neurochemical effects of nicotine. SV2C has a calculated molecular weight of ~82 kDa, but is detected at ~120 kDa by Western blot. This discrepancy is mainly due to extensive N‑glycosylation.