SYCE1 Polyclonal antibody

SYCE1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11063-1-AP

Synonyms

CT76, Cancer/testis antigen 76, C10orf94

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Validation Data Gallery

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  WB analysis of mouse testis using 11063-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11063-1-AP (SYCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11063-1-AP

  IP result of anti-SYCE1 (IP:11063-1-AP, 4ug; Detection:11063-1-AP 1:800) with mouse testis tissue lysate 4800ug.

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  IHC staining of human ovary tumor using 11063-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11063-1-AP (SYCE1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 11063-1-AP

  Immunofluorescent analysis of HepG2 cells, using SYCE1 antibody 11063-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 4 publications below

Product Information

11063-1-AP targets SYCE1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYCE1 fusion protein Ag1527
• Full Name: synaptonemal complex central element protein 1
• Calculated Molecular Weight: 315 aa, 36 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: BC034821
• Gene Symbol: SYCE1
• Gene ID (NCBI): 93426
• RRID: AB_2197057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Synaptonemal Complex Central Element 1 (Syce1) is a major component of the synaptonemal complex (SC), which is formed between homologous chromosomes during meiotic prophase and exists only during the first meiotic division. The SYCE1 structural core, formed by an N-terminal region of amino acids 25-179, is an α-helical dimer that adopts an anti-parallel "coiled-coil"-like structure of approximately 20 nm in length (PMID: 30607510). The male Syce1 null mice had abundant primary spermatocytes and lacked postmeiotic cells; arrested cells were likely eliminated by accelerated apoptosis ( PMID: 25899990).

Publications

Species	Application	Title
mouse	IF	Nat Commun; C14ORF39/SIX6OS1 is a constituent of the synaptonemal complex and is essential for mouse fertility.; Authors - Laura Gómez-H
mouse	IF	Am J Hum Genet; Homozygous mutations in C14orf39/SIX6OS1 cause non-obstructive azoospermia and premature ovarian insufficiency in humans.; Authors - Suixing Fan
mouse	WB,IF	J Steroid Biochem Mol Biol; Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells.; Authors - Qi Wang
mouse	WB,IF	Cell Rep; Male fertility in mice requires classical and nonclassical androgen signaling.; Authors - Paul S Cooke