SYCE1 Polyclonal antibody

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SYCE1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17406-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

C10orf94, Cancer/testis antigen 76, CT76, SYCE1

• 

  WB analysis of mouse testis using 17406-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17406-1-AP (SYCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat testis using 17406-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17406-1-AP (SYCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 17406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17406-1-AP (SYCE1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 17406-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17406-1-AP (SYCE1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 17406-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17406-1-AP (SYCE1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary using 17406-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17406-1-AP (SYCE1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: human testis tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17406-1-AP targets SYCE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11426

  Product name: Recombinant human SYCE1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-315 aa of BC034821

  Sequence: MEMVQKLQKVGSLEPRVEVLINRINEVQQAKKKANKDLGEARTICEALQKELDSLHGEKVHLKEILSKKQETLRILRLHCQEKESEAHRKHTMLQDCKERISALNLQIEEEKNKQRQLRLAFEEQLEDLMGQHKDLWDFHMPERLAREICALDSSKEQLLKEEKLVKATLEDVKHQLCSLCGAEGPSTLDEGLFLRSQEAAATVQLFQEEHRKAEELLAAAAQRHQQLQQKCQQQQQKRQRLKEELEKHGMQVPAQAQSTQEEEAGPGDVASPKPLKGERPGAARQAGPDVLIGQEDTLHPDLSPRGFQEIKELF

• Full Name: synaptonemal complex central element protein 1
• Calculated Molecular Weight: 315 aa, 36 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC034821
• Gene Symbol: SYCE1
• Gene ID (NCBI): 93426
• RRID: AB_10597113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N0S2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The anti-SYCE1 antibody (Cat# 17406-1-AP) has 3 total citations published in Science Advances, Proceedings of the National Academy of Sciences, and Journal of Cellular and Molecular Medicine. The research fields covered include meiotic chromosome synapsis, human infertility, germ cell differentiation, and sex determination in reptiles. Applications cited include immunofluorescence (IF), Western blot (WB), and immunohistochemistry (IHC) in mouse and human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Sci Adv; Meiotic chromosome synapsis depends on multivalent SYCE1-SIX6OS1 interactions that are disrupted in cases of human infertility.; Authors - Fernando Sánchez-Sáez ; KO Validated
	IF	Proc Natl Acad Sci U S A; Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in Trachemys scripta; Authors - Talia Hatkevich
human	WB,IHC	J Cell Mol Med; Novel exon mutation in SYCE1 gene is associated with non-obstructive azoospermia.; Authors - Ke Feng