SYK Polyclonal antibody

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  WB analysis of HEK-293 using 22206-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22206-1-AP (SYK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22206-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22206-1-AP (SYK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 22206-1-AP

  IP result of anti-SYK (IP:22206-1-AP, 4ug; Detection:22206-1-AP 1:500) with HEK-293 cells lysate 2700ug.

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  IHC staining of human spleen using 22206-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22206-1-AP (SYK Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 22206-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22206-1-AP (SYK Antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Ramos using 22206-1-AP

  1x10^6 Ramos cells were intracellularly stained with 0.4 ug SYK Polyclonal antibody (22206-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

SYK Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 22206-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

EC:2.7.10.2, IMD82, p72-Syk, spleen tyrosine kinase, Tyrosine protein kinase SYK

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

22206-1-AP targets SYK in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17526

  Product name: Recombinant human SYK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 239-427 aa of BC001645

  Sequence: QLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQL

• Full Name: spleen tyrosine kinase
• Calculated Molecular Weight: 635 aa, 72 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC001645
• Gene Symbol: SYK
• Gene ID (NCBI): 6850
• ENSEMBL Gene ID: ENSG00000165025
• RRID: AB_2879028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43405
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYK(spleen tyrosine kinase) is a key regulator of signal transduction events,apoptosis and orderly cell cycle progression in B-lineage lymphoid cells. Although SYK has not been linked to a human disease, defective expression of the closely related T-cell tyrosine kinase ZAP-70 has been associated with severe combined immunodeficiency(PMID:11494125). This protein has 2 isoforms produced by alternative splicing. SYK also promotes liver fibrosis by activation of hepatic stellate cells and acts as a biomarker for human hepatocellular carcinoma. (PMID: 29537660)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Virulence; Complement receptor 3 mediates Aspergillus fumigatus internalization into alveolar epithelial cells with the increase of intracellular phosphatidic acid by activating FAK.; Authors - Xuelin Han
mouse	WB	Biochim Biophys Acta; Syk negatively regulates TLR4-mediated IFNβ and IL-10 production and promotes inflammatory responses in dendritic cells.; Authors - Hui Yin ; KO Validated