SYNGAP1 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85045-1-RR (same clone as 85045-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85045-1-RR (SYNGAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85045-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 85045-1-RR (same clone as 85045-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using SYNGAP1 antibody (85045-1-RR, Clone: 242255E3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85045-1-PBS in a different storage buffer formulation.

SYNGAP1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85045-1-PBS

Clone No.242255E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Synaptic Ras GTPase-activating protein 1, RASA 1, Ras/Rap GTPase-activating protein SynGAP, Ras GTPase activating protein SynGAP, Neuronal RasGAP

---

Product Information

85045-1-PBS targets SYNGAP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: synaptic Ras GTPase activating protein 1 homolog (rat)
• Calculated Molecular Weight: 1343 aa, 148 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_006772
• Gene Symbol: SYNGAP1
• Gene ID (NCBI): 8831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96PV0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SYNGAP1, also named as KIAA1938, is the major constituent of the PSD essential for postsynaptic signaling. It's an inhibitory regulator of the Ras-cAMP pathway. SYNGAP1 is a member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. SYNGAP1 regulates AMPAR-mediated miniature excitatory postsynaptic currents. SYNGAP1 may be involved in certain forms of brain injury, leading to long-term learning and memory deficits Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5).