CoraLite® Plus 488-conjugated SYNGAP1 Recombinant antibody

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  IF Staining of U-251 using CL488-85045

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 SYNGAP1 antibody (CL488-85045, Clone: 242255E3 ) at dilution of 1:200, CL594-Phalloidin (red).

SYNGAP1 Uni-rAb^TM Recombinant Antibody for IF/ICC

Cat No. CL488-85045

Clone No.242255E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

242255E3, Neuronal RasGAP, Ras GTPase activating protein SynGAP, Ras/Rap GTPase-activating protein SynGAP, RASA 1

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Tested Applications

• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85045 targets SYNGAP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: synaptic Ras GTPase activating protein 1 homolog (rat)
• Calculated Molecular Weight: 1343 aa, 148 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_006772
• Gene Symbol: SYNGAP1
• Gene ID (NCBI): 8831
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96PV0
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SYNGAP1, also named as KIAA1938, is the major constituent of the PSD essential for postsynaptic signaling. It's an inhibitory regulator of the Ras-cAMP pathway. SYNGAP1 is a member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. SYNGAP1 regulates AMPAR-mediated miniature excitatory postsynaptic currents. SYNGAP1 may be involved in certain forms of brain injury, leading to long-term learning and memory deficits Defects in SYNGAP1 are the cause of mental retardation autosomal dominant type 5 (MRD5).