Tested Applications
| Positive WB detected in | mouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human colon tissue, human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14143-1-AP targets Synaptoporin in WB, IHC, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5307 Product name: Recombinant human SYNPR protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 15-265 aa of BC022518 Sequence: FATCGGYSGGLRLSVDCVNKTESNLSIDIAFAYPFRLHQVTFEVPTCEGKERQKLALIGDSSSSAEFFVTVAVFAFLYSLAATVVYIFFQNKYRENNRGPLIDFIVTVVFSFLWLVGSSAWAKGLSDVKVATDPKEVLLLMSACKQPSNKCMAIHSPVMSSLNTSVVFGFLNFILWAGNIWFVFKETGWHSSGQRYLSDPMEKHSSSYNQGGYNQDSYGSSSGYSQQASLGPTSDEFGQQPTGPTSFTNQI Predict reactive species |
| Full Name | synaptoporin |
| Calculated Molecular Weight | 265 aa, 29 kDa |
| Observed Molecular Weight | 37 kDa |
| GenBank Accession Number | BC022518 |
| Gene Symbol | Synaptoporin |
| Gene ID (NCBI) | 132204 |
| RRID | AB_2878022 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TBG9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Synaptoporin antibody 14143-1-AP | Download protocol |
| IHC protocol for Synaptoporin antibody 14143-1-AP | Download protocol |
| IP protocol for Synaptoporin antibody 14143-1-AP | Download protocol |
| WB protocol for Synaptoporin antibody 14143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Synaptoporin Polyclonal antibody (Cat# 14143-1-AP) has 2 citations. They appear in CNS Neuroscience & Therapeutics (2023) and Biomedicines (2022), both using immunofluorescence in mouse models. The research fields span epilepsy and synaptic signaling (ANXA2/GluA1 phosphorylation) and Fragile X syndrome-related hippocampal dendritic spine dysgenesis.
| Species | Application | Title |
|---|---|---|
CNS Neurosci Ther Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation | ||
Biomedicines Combined DiI and Antibody Labeling Reveals Complex Dysgenesis of Hippocampal Dendritic Spines in a Mouse Model of Fragile X Syndrome |
Reviews
What customers say
The single review for this antibody is highly positive, rated 5 stars. The customer used it for immunofluorescence on myenteric plexus of adult mouse intestine at a 1:300 dilution and described it as a "great antibody." Overall, the feedback is favorable, indicating reliable performance in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cajsa (Verified Customer) (10-07-2024) | Great antibody!
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