Synaptotagmin-12 Polyclonal antibody

Synaptotagmin-12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55015-1-AP

Synonyms

SRG1, Synaptotagmin 12, synaptotagmin XII, Synaptotagmin-12, SYT12, SytXII

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Validation Data Gallery

• 

  WB analysis of mouse brain using 55015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55015-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55015-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 55015-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

55015-1-AP targets Synaptotagmin-12 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: synaptotagmin XII
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: NM_177963
• Gene Symbol: Synaptotagmin-12
• Gene ID (NCBI): 91683
• RRID: AB_10859791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYT12 belongs to the synaptotagmin family. It may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. The antibody is specific to STY12.

Publications

Species	Application	Title
mouse	WB	Oxid Med Cell Longev; Proteomic Profile of Mouse Brain Aging Contributions to Mitochondrial Dysfunction, DNA Oxidative Damage, Loss of Neurotrophic Factor, and Synaptic and Ribosomal Proteins.; Authors - Yingchao Li
mouse	WB,IF	Nat Neurosci; Adaptor protein AP-3 produces synaptic vesicles that release at high frequency by recruiting phospholipid flippase ATP8A1; Authors - Hongfei Xu