|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12128-1-AP targets SYTL4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SYTL4 fusion protein Ag2772|
|Full Name||synaptotagmin-like 4|
|Calculated molecular weight||671 aa, 76 kDa|
|Observed molecular weight||76 kDa|
|GenBank accession number||BC014913|
|Gene ID (NCBI)||94121|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SYTL4, also known as SLP4 or granuphilin, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL4 is specifically expressed on dense-core granules in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. (PMID: 16481396; 16473610)
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J Proteome Res
Identification of tyrosine-phosphorylated proteins associated with lung cancer metastasis using label-free quantitative analyses.
SYTL4 downregulates microtubule stability and confers paclitaxel resistance in triple-negative breast cancer.
Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.