SYTL4 Polyclonal antibody

SYTL4 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12128-1-AP

Synonyms

Exophilin 2, Granuphilin, SLP4, synaptotagmin like 4, Synaptotagmin like protein 4, SYTL4



Tested Applications

Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12128-1-AP targets SYTL4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SYTL4 fusion protein Ag2772
Full Name synaptotagmin-like 4
Calculated molecular weight 671 aa, 76 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC014913
Gene symbol SYTL4
Gene ID (NCBI) 94121
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYTL4, also known as SLP4 or granuphilin, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL4 is specifically expressed on dense-core granules in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. (PMID: 16481396; 16473610)

Protocols

Product Specific Protocols
IHC protocol for SYTL4 antibody 12128-1-APDownload protocol
IP protocol for SYTL4 antibody 12128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Arterioscler Thromb Vasc Biol

Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.

Authors - Frédéric Adam
ratWB

Biophys Rep

PMID: 30596141

Authors - Min Li
humanWB

J Proteome Res

Identification of tyrosine-phosphorylated proteins associated with lung cancer metastasis using label-free quantitative analyses.

Authors - Wu Hsin-Yi HY
humanWB,IF

Theranostics

SYTL4 downregulates microtubule stability and confers paclitaxel resistance in triple-negative breast cancer.

Authors - Xi-Yu Liu
  • KD Validated
humanWB

Nat Commun

Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.

Authors - Akiko Takahashi