SYTL4 Polyclonal antibody

SYTL4 Polyclonal Antibody for IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12128-1-AP

Synonyms

Exophilin 2, Granuphilin, SLP4, synaptotagmin like 4, Synaptotagmin like protein 4, SYTL4

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Validation Data Gallery

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  IP experiment of HEK-293 cells using 12128-1-AP

  IP result of anti-SYTL4 (IP:12128-1-AP, 4ug; Detection:12128-1-AP 1:300) with HEK-293 cells lysate 1600 ug.

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  IHC staining of human breast cancer tissue using 12128-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12128-1-AP (SYTL4 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IP: See 1 publications below

Product Information

12128-1-AP targets SYTL4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYTL4 fusion protein Ag2772
• Full Name: synaptotagmin-like 4
• Calculated molecular weight: 671 aa, 76 kDa
• Observed molecular weight: 76 kDa
• GenBank accession number: BC014913
• Gene symbol: SYTL4
• Gene ID (NCBI): 94121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SYTL4, also known as SLP4 or granuphilin, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL4 is specifically expressed on dense-core granules in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. (PMID: 16481396; 16473610)

Publications

Species	Application	Title
human	WB,IP	Arterioscler Thromb Vasc Biol; Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.; Authors - Frédéric Adam
rat	WB	Biophys Rep; PMID: 30596141; Authors - Min Li
human	WB	J Proteome Res; Identification of tyrosine-phosphorylated proteins associated with lung cancer metastasis using label-free quantitative analyses.; Authors - Wu Hsin-Yi HY
human	WB	Nat Commun; Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.; Authors - Akiko Takahashi