SYTL4 Polyclonal antibody

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  WB analysis using 12128-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12128-1-AP (SYTL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12128-1-AP

  mouse pancreas tissue was subjected to SDS PAGE followed by western blot with 12128-1-AP (SYTL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12128-1-AP

  IP result of anti-SYTL4 (IP:12128-1-AP, 4ug; Detection:12128-1-AP 1:300) with HEK-293 cells lysate 1600 ug.

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  IHC staining of human breast cancer using 12128-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12128-1-AP (SYTL4 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 12128-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12128-1-AP (SYTL4 antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

SYTL4 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12128-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Exophilin 2, Granuphilin, SLP4, synaptotagmin like 4, Synaptotagmin like protein 4, SYTL4

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Tested Applications

• Positive WB detected in: HeLa cells, mouse pancreas tissue, U2OS cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12128-1-AP targets SYTL4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SYTL4 fusion protein Ag2772
• Full Name: synaptotagmin-like 4
• Calculated Molecular Weight: 671 aa, 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC014913
• Gene Symbol: SYTL4
• Gene ID (NCBI): 94121
• RRID: AB_2877828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96C24
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SYTL4, also known as SLP4 or granuphilin, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL4 is specifically expressed on dense-core granules in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. (PMID: 16481396; 16473610)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.; Authors - Akiko Takahashi
human	WB,IP	Arterioscler Thromb Vasc Biol; Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.; Authors - Frédéric Adam
human	WB,IF	Theranostics; SYTL4 downregulates microtubule stability and confers paclitaxel resistance in triple-negative breast cancer.; Authors - Xi-Yu Liu ; KD Validated
human	WB	J Proteome Res; Identification of tyrosine-phosphorylated proteins associated with lung cancer metastasis using label-free quantitative analyses.; Authors - Wu Hsin-Yi HY
rat	WB	Biophys Rep; PMID: 30596141; Authors - Min Li
rat	WB	Cell Commun Signal; Nesfatin-1 enhances vascular smooth muscle calcification through facilitating BMP-2 osteogenic signaling; Authors - Xue-Xue Zhu ; KD Validated