SYTL4 Polyclonal antibody
SYTL4 Polyclonal Antibody for IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12128-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
12128-1-AP targets SYTL4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SYTL4 fusion protein Ag2772 |
Full Name | synaptotagmin-like 4 |
Calculated molecular weight | 671 aa, 76 kDa |
Observed molecular weight | 76 kDa |
GenBank accession number | BC014913 |
Gene symbol | SYTL4 |
Gene ID (NCBI) | 94121 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SYTL4, also known as SLP4 or granuphilin, is a member of the synaptotagmin-like protein (Slp) family. Members of this family are characterized by presence of an N-terminal Slp homology domain (SHD), which functions as a Rab27-binding domain, and C-terminal tandem C2 domains, putative Ca(2+)-binding motifs. SYTL4 is specifically expressed on dense-core granules in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. (PMID: 16481396; 16473610)
Protocols
Product Specific Protocols | |
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IHC protocol for SYTL4 antibody 12128-1-AP | Download protocol |
IP protocol for SYTL4 antibody 12128-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Arterioscler Thromb Vasc Biol Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability. | ||
J Proteome Res Identification of tyrosine-phosphorylated proteins associated with lung cancer metastasis using label-free quantitative analyses. | ||
Theranostics SYTL4 downregulates microtubule stability and confers paclitaxel resistance in triple-negative breast cancer.
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