RISC Monoclonal antibody

RISC Monoclonal Antibody for WB, IF, IHC, ELISA

Cat No. 60086-1-Ig

Clone No. 1C6A7

Publications (1)

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Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

2410018F01Rik, 4833411K15Rik, AI957256, Risc, Scpep1, serine carboxypeptidase 1

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

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Filter: AllWBIHCIF

WB analysis of human testis using 60086-1-Ig
human testis tissue were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 60086-1-Ig
MCF7 cells were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 60086-1-Ig
MCF7 cells were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human heart using 60086-1-Ig
human heart tissue were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 60086-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60086-1-Ig (RISC antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas cancer using 60086-1-Ig
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60086-1-Ig (RISC antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of C6 using 60086-1-Ig
Immunofluorescent analysis of C6 cells using 60086-1-Ig (RISC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)

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MSDS SDS

Tested Applications

Positive WB detected in	human testis tissue, human heart tissue, MCF-7 cells
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	C6 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60086-1-Ig targets RISC in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag0614 Product name: Recombinant mouse Scpep1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-280 aa of AF330052 Sequence: AAGISVELYKAVQQGTIKCNFSGVALGDSWISPVDSVLSWGPYLYSMSLLDNQGLAMVSDIAEQVLDAVNKGFYKEATQLWGKAEMIIEKNTDGVNFYNILTKSSPEKAMESSLEFLRSPLVRLCQRHVRHLQGDALSQLMNGPIKKKLKIIPEDISWGAQASYVFLSMEGDFMKPAIDVVDKLLAAGVNVTVYNGQLDLIVDTIGQESWVQKLKWPQLSKFNQLKWKALYTDPKSSETAAFVKSYENLAFYWILKAGHMVPSDQGEMALKMMKLVTKQE Predict reactive species
Full Name	serine carboxypeptidase 1
Calculated Molecular Weight	452 aa, 51 kDa
Observed Molecular Weight	35-40 kDa/50-60 kDa
GenBank Accession Number	AF330052
Gene Symbol	Scpep1
Gene ID (NCBI)	74617
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q920A5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Scpep1(Retinoid-inducible serine carboxypeptidase) is a 51 kDa protein which is also named as RISC and belongs to the peptidase S10 family. This protein may be involved in vascular wall and kidney homeostasis.

Publications

What published studies show

The RISC Monoclonal antibody (1C6A7), catalog number 60086-1-Ig, has 1 total citation. The cited publication appeared in Autophagy (Impact Factor 18.6), published in January 2020. The study investigated how donor mesenchymal stem cells release apoptotic bodies to improve myocardial infarction via autophagy regulation in recipient cells. The antibody was used for Western blot in human samples. The associated research fields include autophagy, cardiac injury/myocardial infarction, and mesenchymal stem cell biology.

Species	Application	Title
human	WB	Autophagy Donor MSCs release apoptotic bodies to improve myocardial infarction via autophagy regulation in recipient cells. Authors - Huan Liu View Article