Serum amyloid P component Recombinant monoclonal antibody

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• Serum amyloid P component Recombinant monoclonal antibody (84402-1-RR)

Serum amyloid P component Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84402-1-RR Copied!

Clone No. 241403F5

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

APCS, SAP, 241403F5, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein

• 

  WB analysis of human plasma using 84402-1-RR

  human plasma was subjected to SDS PAGE followed by western blot with 84402-1-RR (Serum amyloid P component antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 84402-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84402-1-RR against Human Serum amyloid P component were performed. The affinity constant is 1.49 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human plasma

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84402-1-RR targets Serum amyloid P component in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0528

  Product name: Recombinant Human Serum amyloid P component /APCS protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 20-223 aa of BC007058

  Sequence: HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV

• Full Name: amyloid P component, serum
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 27-29 kDa
• GenBank Accession Number: BC007058
• Gene Symbol: Serum amyloid P component
• Gene ID (NCBI): 325
• RRID: AB_3671931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P02743
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serum amyloid P component (SAP), a member of the pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand-binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.