Serum amyloid P component Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis of human plasma using 84402-1-RR (same clone as 84402-1-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 84402-1-RR (Serum amyloid P component antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84402-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01275-1

  Sandwich ELISA standard curve of MP01275-1, Human Serum amyloid P component Recombinant Matched Antibody Pair - PBS only. 84402-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0528. 84402-1-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL

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  Sample test of MP01275-1

  Serum of eight individual healthy human donors was measured. The human Serum amyloid P component concentration of detected samples was determined to be 32.91 µg/mL with a range of 16.86 - 48.68 µg/mL.

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  Affinity and Kinetic Characterization of 84402-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84402-1-RR against Human Serum amyloid P component were performed. The affinity constant is 1.49 nM.

Serum amyloid P component Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84402-1-PBS

Clone No.241403F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

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Product Information

84402-1-PBS targets Serum amyloid P component as part of a matched antibody pair:

MP01275-1: 84402-2-PBS capture and 84402-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: amyloid P component, serum
• Calculated Molecular Weight: 223 aa, 25 kDa
• Observed Molecular Weight: 27-29 kDa
• GenBank Accession Number: BC007058
• Gene Symbol: Serum amyloid P component
• Gene ID (NCBI): 325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P02743
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Serum amyloid P component (SAP), a member of the pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand-binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.