Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF Staining of NIH/3T3 using 83280-1-RR
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Shh antibody (83280-1-RR, Clone: 240057E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Shh antibody (83280-1-RR, Clone: 240057E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Affinity and Kinetic Characterization of 83280-1-RR
Biolayer interferometry (BLl) kinetic assays of 83280-1-RR against Mouse SHH were performed. The affinity constant is below 1 pM.
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Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IF/ICC Figures
IF Staining of NIH/3T3 using 83280-1-RR
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Shh antibody (83280-1-RR, Clone: 240057E7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
AFFINITY Figures
Affinity and Kinetic Characterization of 83280-1-RR
Biolayer interferometry (BLl) kinetic assays of 83280-1-RR against Mouse SHH were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Shh Recombinant antibody
Catalog Number
83280-1-RR
Citations
-
Dilutions
WB : 1:5000-1:50000 IF/ICC : 1:125-1:500
Applications
WB, IF/ICC, ELISA
Reactivity
mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
