Siglec-15 Polyclonal antibody

Siglec-15 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 28594-1-AP


CD33 antigen like 3, CD33L3, HsT1361, SIGLEC 15, SIGLEC15

Tested Applications

Positive WB detected inPC-3 cells, U-87 MG cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
Sample-dependent, check data in validation data gallery

Product Information

28594-1-AP targets Siglec-15 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Siglec-15 fusion protein Ag29829
Full Name sialic acid binding Ig-like lectin 15
Calculated molecular weight 36 kDa
Observed molecular weight38-40 kDa
GenBank accession numberNM_213602
Gene symbol SIGLEC15
Gene ID (NCBI) 284266
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sialic acid binding Ig-like lectin 15 (Siglec-15) is a member of the Siglec family of glycan-recognition proteins. Siglec-15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain, and a short cytoplasmic tail (PMID: 17483134). It is mainly expressed on a subset of myeloid lineage cells and can be upregulated in many cancers (PMID: 32939323). Siglec-15 has been reported as a immune suppressor and a potential target for normalization cancer immunotherapy (PMID: 30833750). Siglec-15 has a calculated molecular weight of 36 kDa. It has been detected at 38-40 kDa due to glycosylation (PMID: 32921411).


Product Specific Protocols
WB protocol for Siglec-15 antibody 28594-1-APDownload protocol
Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Rebecca (Verified Customer) (07-21-2022)

Positive staining identified at 1:500 dilution in patient FFPE tissue. Slight background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Prostate cancer FFPE tissue
Siglec-15 Antibody Immunofluorescence validation (1:500 dilution) in Prostate cancer FFPE tissue (Cat no:28594-1-AP)