Anti-Human Siglec-8 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of human peripheral blood leukocytes using 98177-1-RR (same clone as 98177-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-8 Rabbit Recombinant Antibody (98177-1-RR, Clone: 241275A5) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16. Cells were not fixed. This data was developed using the same antibody clone with 98177-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98177-1-RR (same clone as 98177-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Siglec-8 Rabbit Recombinant Antibody (98177-1-RR, Clone: 241275A5) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98177-1-PBS in a different storage buffer formulation.

Siglec-8 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98177-1-PBS

Clone No.241275A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIGLEC8, SIGLEC 8, Sialoadhesin family member 2, Sialic acid-binding Ig-like lectin 8, SAF2

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Product Information

98177-1-PBS targets Siglec-8 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: sialic acid binding Ig-like lectin 8
• Calculated Molecular Weight: 499 aa, 54 kDa
• GenBank Accession Number: BC053319
• Gene Symbol: SIGLEC8
• Gene ID (NCBI): 27181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NYZ4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Sialic acid-binding Ig-like lectin 8 (Siglec-8), also known as SAF2, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 10856141). Siglec-8 is a single-pass transmembrane protein containing three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail (PMID: 10625619). It is an inhibitory receptor selectively expressed on human mast cells and eosinophils, and at low levels on basophils (PMID: 10856141; 33374255). Engagement of Siglec-8 with antibody results in inhibiting IgE-mediated mast cell responses and reducing allergic inflammation (PMID: 32814824).