FcZero-rAb® APC Anti-Human Siglec-8 Rabbit Recombinant Antibody

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• FcZero-rAb® APC Anti-Human Siglec-8 Rabbit Recombinant Antibody (APC-FcA98177)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

Siglec-8 FcZero-rAb^® Recombinant Antibody for FC

Cat No. APC-FcA98177 Copied!

Clone No. 241275A5

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIGLEC8, SAF 2, SAF2, Sialic acid-binding Ig-like lectin 8, Sialoadhesin family member 2

• 

  FC experiment of human peripheral blood leukocyte using APC-FcA98177

  1x10^6 human peripheral blood leukocytes were surface stained with FcZero-rAb™ PE Anti-Human CD16, and 5 ul APC Anti-Human Siglec-8 Rabbit RecAb (APC-FcA98177, Clone: 241275A5) or APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human peripheral blood leukocyte using APC-FcA98177

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul APC Anti-Human Siglec-8 Rabbit RecAb (APC-FcA98177, Clone: 241275A5), or APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

APC-FcA98177 targets Siglec-8 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: sialic acid binding Ig-like lectin 8
• Calculated Molecular Weight: 499 aa, 54 kDa
• GenBank Accession Number: BC053319
• Gene Symbol: SIGLEC8
• Gene ID (NCBI): 27181
• RRID: AB_3746580
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NYZ4
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Sialic acid-binding Ig-like lectin 8 (Siglec-8), also known as SAF2, is a sialic acid-binding immunoglobulin-like lectin that belongs to the SIGLEC family of the Ig superfamily (PMID: 10856141). Siglec-8 is a single-pass transmembrane protein containing three extracellular immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tail (PMID: 10625619). It is an inhibitory receptor selectively expressed on human mast cells and eosinophils, and at low levels on basophils (PMID: 10856141; 33374255). Engagement of Siglec-8 with antibody results in inhibiting IgE-mediated mast cell responses and reducing allergic inflammation (PMID: 32814824).