Smoothelin Recombinant monoclonal antibody, PBS Only

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• Smoothelin Recombinant monoclonal antibody, PBS Only (87674-7-PBS)

• 

  WB analysis using 87674-7-RR (same clone as 87674-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87674-7-PBS in a different storage buffer formulation.

• 

  WB analysis using 87674-7-RR (same clone as 87674-7-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87674-7-PBS in a different storage buffer formulation.

• 

  WB analysis using 87674-7-RR (same clone as 87674-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87674-7-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 87674-7-RR (same clone as 87674-7-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87674-7-PBS in a different storage buffer formulation.

• 

  Affinity of 87674-7-RR

  Biolayer interferometry (BLI) kinetic assays of 87674-7-RR against Human Smoothelin were performed. The affinity constant is 0.257 nM.

Smoothelin Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87674-7-PBS

Clone No.253233C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

SMTN, SMSMO

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Product Information

87674-7-PBS targets Smoothelin in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19983

  Product name: Recombinant human SMTN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 458-811 aa of BC034237

  Sequence: KTTFTIEIKDGRGQASTGRVLLPTGNQRAELTLGLRAPPTLLSTSSGGKSTITRVNSPGTLARLGSVTHVTSFSHAPPSSRGGCSIKMEPEPAEPLAAAVEAANGAEQTRVNKAPEGRSPLSAEELMTIEDEGVLDKMLDQSTDFEERKLIRAALRELRQRKRDQRDKERERRLQEARGRPGEGRGNTATETTTRHSQRAADGSAVSTVTKTERLVHSNDGTRTARTTTVESSFVRRSENGSGSTMMQTKTFSSSSSSKKMGSIFDREDQASPRAGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGAAGSPGGPRAAVQRSTSFGVPNANSIKQMLLDWCRA

• Full Name: smoothelin
• Calculated Molecular Weight: 940 aa, 102 kDa
• Observed Molecular Weight: 59 kDa and 117 kDa
• GenBank Accession Number: BC034237
• Gene Symbol: SMTN
• Gene ID (NCBI): 6525
• RRID: AB_3745689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P53814
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Smoothelin is a cytoskeletal-associated protein expressed specifically in differentiated smooth muscle cells (SMCs). It exists in two main isoforms: the ~59 kDa Smoothelin-A in visceral SMCs and the ~110 kDa Smoothelin-B in vascular SMCs, generated through a dual-promoter system and alternative splicing. Smoothelin serves as a reliable marker of contractile SMCs and helps maintain the contractile phenotype by interacting with actin filaments and other cytoskeletal components. This antibody reacts with the 59 kDa and 117 kDa bands, corresponding to isoforms A and B, respectively.