Smoothelin Recombinant monoclonal antibody

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• Smoothelin Recombinant monoclonal antibody (87674-7-RR)

• 

  WB analysis using 87674-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87674-7-RR

  PC-3 cells were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87674-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 87674-7-RR

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 87674-7-RR (Smoothelin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity of 87674-7-RR

  Biolayer interferometry (BLI) kinetic assays of 87674-7-RR against Human Smoothelin were performed. The affinity constant is 0.257 nM.

Smoothelin Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87674-7-RR

Clone No.253233C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

SMTN, SMSMO

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Tested Applications

• Positive WB detected in: 5637 cells, COLO 320 cells, mouse colon tissue, PC-3 cells, MCF-7 cells, rat colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87674-7-RR targets Smoothelin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19983

  Product name: Recombinant human SMTN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 458-811 aa of BC034237

  Sequence: KTTFTIEIKDGRGQASTGRVLLPTGNQRAELTLGLRAPPTLLSTSSGGKSTITRVNSPGTLARLGSVTHVTSFSHAPPSSRGGCSIKMEPEPAEPLAAAVEAANGAEQTRVNKAPEGRSPLSAEELMTIEDEGVLDKMLDQSTDFEERKLIRAALRELRQRKRDQRDKERERRLQEARGRPGEGRGNTATETTTRHSQRAADGSAVSTVTKTERLVHSNDGTRTARTTTVESSFVRRSENGSGSTMMQTKTFSSSSSSKKMGSIFDREDQASPRAGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGAAGSPGGPRAAVQRSTSFGVPNANSIKQMLLDWCRA

• Full Name: smoothelin
• Calculated Molecular Weight: 940 aa, 102 kDa
• Observed Molecular Weight: 59 kDa and 117 kDa
• GenBank Accession Number: BC034237
• Gene Symbol: SMTN
• Gene ID (NCBI): 6525
• RRID: AB_3745689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P53814
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Smoothelin is a cytoskeletal-associated protein expressed specifically in differentiated smooth muscle cells (SMCs). It exists in two main isoforms: the ~59 kDa Smoothelin-A in visceral SMCs and the ~110 kDa Smoothelin-B in vascular SMCs, generated through a dual-promoter system and alternative splicing. Smoothelin serves as a reliable marker of contractile SMCs and helps maintain the contractile phenotype by interacting with actin filaments and other cytoskeletal components. This antibody reacts with the 59 kDa and 117 kDa bands, corresponding to isoforms A and B, respectively.