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CoraLite® Plus 488-conjugated Sortilin Monoclonal antibody

Sortilin Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B11B9

Cat no : CL488-68007

Synonyms

100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3, SORT1, Sortilin, sortilin 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Sortilin Monoclonal (CL488-68007)

    IF Staining of mouse brain using CL488-68007

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Sortilin antibody (CL488-68007, Clone: 2B11B9 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated Sortilin Monoclonal (CL488-68007)

    IF Staining of mouse brain using CL488-68007

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Sortilin antibody (CL488-68007, Clone: 2B11B9 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 488-conjugated Sortilin Monoclonal (CL488-68007)

    FC experiment of SH-SY5Y using CL488-68007

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Sortilin (CL488-68007, Clone:2B11B9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inmouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68007 targets Sortilin in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Sortilin fusion protein Ag28043
Full Name sortilin 1
Calculated Molecular Weight 831 aa, 92 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC023542
Gene Symbol SORT1
Gene ID (NCBI) 6272
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Sortilin antibody CL488-68007Download protocol
FC protocol for CL Plus 488 Sortilin antibody CL488-68007Download protocol
Standard Protocols
Click here to view our Standard Protocols