ChromoTek Spot-Trap® Magnetic Particles M-270

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

Specificity

Spot-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Particles M-270

Cat no : etdk

Synonyms

etdk, etdk-20, Spot Trap Magnetic Particles M-270, Spot-Trap Magnetic Particles M-270, Spot-Trap Magnetic Particles M-270 Kit, Spot-Trap® Magnetic Particles M-270 Kit, etdk-20, PDRVRAVSHWSS, spot, spot tag, spot-tag, Spot-Trap® Magnetic Particles M-270



Product Information

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-Tag sequence motif PDRVRAVSHWSS
GeneID (NCBI)0
Coupled VHHRecombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 2.8 µm (Magnetic, high throughput-compatible)
Binding capacity10 µL slurry bind about 0.9 µg of recombinant Spot-Tag fusion protein (30 kDa)
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Storage Buffer1x PBS, Preservative: 0.09 % sodium azide
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Spot-Trap Magnetic Particles M-27020 reactions (500 µL)