ChromoTek Spot-Trap® Agarose, kit

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

Specificity

Spot-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : etak

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap



Product Information

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-Tag sequence PDRVRAVSHWSS
Coupled VHHRecombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment
ConjugateUnconjugated
Beads~ 40 µm
Binding capacity10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa)
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
Spot-Trap® Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate