ChromoTek Spot-Trap® Magnetic Agarose

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

Specificity

Spot-Tag^®

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Publications

7

Cat no : etma

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap

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Validation Data Gallery

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  Immunoprecipitation of Spot-tagged protein.

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  Spot-Trap Magnetic Agarose for immunoprecipitation of Spot-tagged proteins: Coomassie blue stained gel and Western blot I: Input, FT: Flow-Through, B: Bound.

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  Application of Spot-tag related products.

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Product Information

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

• Description: Immunoprecipitation of Spot-tagged proteins and their interacting factors with anti-Spot Nanobody conjugated to Magnetic Agarose.

  • Magnetic separation with easy and efficient washing of Spot-Trap Magnetic Agarose

  • IP and Co-IP of Spot-tagged proteins without size limitation

  • No contaminating heavy and light antibody chains

  • Suitable for downstream mass spec analysis

  • Recombinantly expressed without batch-to-batch variation

  • Well-characterized and validated

  • Ready-to-use

  • Short incubation time of 30-60 minutes

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: Spot-Tag^® sequence PDRVRAVSHWSS
• Binding capacity: 17.5 μg of recombinant Spot-tagged protein (~30 kDa) per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer; 10 mM NaOH pH 12
• Wash buffer compatibility: 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.1 % SDS
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 6 nM
• Compatibility with mass spectrometry: The Spot-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827591
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Other Formats

Spot-Trap Agarose

Spot-Trap Agarose kit

Spot-Trap Magnetic Agarose kit

Spot-Trap Magnetic Particles M-270

Spot-Trap Magnetic Particles M-270 Kit

Publications

Application	Title
IP	Mol Cell; Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane.; Authors - Masahiro Maruoka
IP	Nucleic Acids Res; Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay.; Authors - Giuditta Annibaldis
IP	Cells; The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection.; Authors - Mohamed Ibrahem Elhawy
IP	J Biol Chem; BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING).; Authors - Kou Motani
	Mol Carcinog; Detection of rapidly accumulating stress-induced SUMO in prostate cancer cells by a fluorescent SUMO biosensor.; Authors - Rui Yin
IP	Life Sci Alliance; The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair.; Authors - Kenji Shimada