ChromoTek Spot-Trap® Magnetic Agarose
Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.
Ready to use reagent
Specificity
Spot-Tag
Applications
IP, CoIP, ChIP, RIP
Conjugate
Magnetic Agarose
Cat no : etma
Synonyms
Validation Data Gallery
Product Information
Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 6 nM |
Specificity/Target | Spot-Tag sequence motif PDRVRAVSHWSS |
Coupled VHH | Recombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment |
Conjugate | Magnetic Agarose |
Beads | ~ 40 µm |
Binding capacity | 10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa) |
Elution buffer | SDS sample buffer 10 mM NaOH pH 12 |
Storage Buffer | 20% ethanol |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS Spot-Trap Magnetic Agarose (PDF) |
Application notes |
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Applications note Spot-System for for CRISPR’ed Spot-Tag |
Product flyer |
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Spot-Tag, Spot-Label & Spot-Trap (PDF) |
Protocol for immunoprecipitation |
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Protocol Spot-Trap Magnetic Agarose (PDF) |
Troubleshooting |
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Troubleshooting guide immunoprecipitation (PDF) |
Publications
Application | Title |
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J Biol Chem BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING). | |
Nucleic Acids Res Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay. |