ChromoTek Spot-Trap® Magnetic Agarose

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

Specificity

Spot-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Publications

6

Cat no : etma

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap

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Validation Data Gallery

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  Immunoprecipitation of Spot-tagged protein.

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  Spot-Trap Magnetic Agarose for immunoprecipitation of Spot-tagged proteins: Coomassie blue stained gel and Western blot I: Input, FT: Flow-Through, B: Bound.

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  Application of Spot-tag related products.

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Product Information

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 6 nM
• Specificity/Target: Spot-Tag sequence motif PDRVRAVSHWSS
• Coupled VHH: Recombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment
• Conjugate: Unconjugated
• Beads: ~ 40 µm
• Binding capacity: 10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa)
• Elution buffer: SDS sample buffer; 10 mM NaOH pH 12
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	Life Sci Alliance; The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair.; Authors - Kenji Shimada
IP	Life Sci Alliance; Cell-autonomous Toxoplasma killing program requires Irgm2 but not its microbe vacuolar localization.; Authors - Ariel Pradipta
IP	J Biol Chem; BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING).; Authors - Kou Motani
IP	Cells; The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection.; Authors - Mohamed Ibrahem Elhawy
IP	Nucleic Acids Res; Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay.; Authors - Giuditta Annibaldis
IP	Mol Cell; Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane.; Authors - Masahiro Maruoka