ChromoTek Spot-Trap® Magnetic Agarose

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

Ready to use reagent

Specificity

Spot-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Publications

2

Cat no : etma

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap

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Validation Data Gallery

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  Immunoprecipitation of Spot-tagged protein.

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  Spot-Trap Magnetic Agarose for immunoprecipitation of Spot-tagged proteins: Coomassie blue stained gel and Western blot I: Input, FT: Flow-Through, B: Bound.

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  Application of Spot-tag related products.

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Product Information

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 6 nM
• Specificity/Target: Spot-Tag sequence motif PDRVRAVSHWSS
• Coupled VHH: Recombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment
• Conjugate: Magnetic Agarose
• Beads: ~ 40 µm
• Binding capacity: 10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa)
• Elution buffer: SDS sample buffer; 10 mM NaOH pH 12
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	J Biol Chem; BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING).; Authors - Kou Motani
IP	Nucleic Acids Res; Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay.; Authors - Giuditta Annibaldis