ChromoTek Spot-Trap® Magnetic Agarose
Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.
Specificity
Spot-Tag
Applications
IP, CoIP, ChIP, RIP
Conjugate
Magnetic Agarose
Cat no : etma
Synonyms
Validation Data Gallery
Product Information
Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.
| Applications | IP, CoIP, ChIP, RIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 6 nM |
| Specificity/Target | Spot-Tag sequence motif PDRVRAVSHWSS |
| Coupled VHH | Recombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment |
| Conjugate | Magnetic Agarose |
| Beads | ~ 40 µm |
| Binding capacity | 10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa) |
| Elution buffer | SDS sample buffer 10 mM NaOH pH 12 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
| SDS |
|---|
| SDS Spot-Trap Magnetic Agarose (PDF) |
| Troubleshooting |
|---|
| Troubleshooting guide immunoprecipitation (PDF) |
| Protocol for immunoprecipitation |
|---|
| Protocol Spot-Trap Magnetic Agarose (PDF) |
| Product flyer |
|---|
| Spot-Tag, Spot-Label & Spot-Trap (PDF) |
| Application notes |
|---|
| Applications note Spot-System for for CRISPR’ed Spot-Tag |
Publications
| Application | Title |
|---|---|
Life Sci Alliance The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair. | |
Life Sci Alliance Cell-autonomous Toxoplasma killing program requires Irgm2 but not its microbe vacuolar localization. | |
Mol Carcinog Detection of rapidly accumulating stress-induced SUMO in prostate cancer cells by a fluorescent SUMO biosensor. | |
J Biol Chem BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING). | |
Cells The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection. | |
Nucleic Acids Res Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay. |