ChromoTek Spot-Trap® Magnetic Agarose

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

Specificity

Spot-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic Agarose

Cat no : etma

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap



Product Information

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality VHH
HostAlpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-Tag sequence motif PDRVRAVSHWSS
Coupled VHHRecombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment
ConjugateMagnetic Agarose
Beads~ 40 µm
Binding capacity10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa)
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Life Sci Alliance

The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair.

Authors - Kenji Shimada
IP

J Biol Chem

BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING).

Authors - Kou Motani
IP

Cells

The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection.

Authors - Mohamed Ibrahem Elhawy
IP

Nucleic Acids Res

Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay.

Authors - Giuditta Annibaldis
IP

Mol Cell

Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane.

Authors - Masahiro Maruoka