ChromoTek Spot-Trap® Magnetic Particles M-270 Kit
Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.
Cat no : etdk
Synonyms
Validation Data Gallery
Product Information
Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.
| Applications | IP, CoIP, ChIP, RIP |
| Clonality | Monoclonal |
| Host | Alpaca |
| Affinity | Dissociation constant KD of 6 nM |
| Specificity/Target | Spot-Tag sequence motif PDRVRAVSHWSS |
| GeneID (NCBI) | 0 |
| Coupled VHH | Recombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment |
| Conjugate | Magnetic Particles M-270 |
| Beads | ~ 2.8 µm (Magnetic, high throughput-compatible) |
| Binding capacity | 10 µL slurry bind about 0.9 µg of recombinant Spot-Tag fusion protein (30 kDa) |
| Elution buffer | SDS sample buffer 10 mM NaOH pH 12 |
| Storage Buffer | 1x PBS, Preservative: 0.09 % sodium azide |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| Lysis buffer | Optimized for cytoplasmatic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Spot-Trap Magnetic Particles M-270 | 20 reactions (500 µL) |
Documentation
| SDS |
|---|
| SDS Spot-Trap Magnetic Particles M-270 Kit |
| PROTOCOL |
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| PROTOCOL Spot-Trap Magnetic Particles M-270 Kit |
| Poster |
|---|
| Scientific Spot system poster |
| Troubleshooting |
|---|
| Troubleshooting guide immunoprecipitation (PDF) |
| Product flyer |
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| Spot-Tag, Spot-Label & Spot-Trap (PDF) |
| Application notes |
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| Applications note Spot-System for for CRISPR’ed Spot-Tag |