ChromoTek Spot-Trap® Magnetic Particles M-270, Kit for Immunoprecipitation

Validation Data Gallery

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  Spot-Trap Magnetic Particles M-270 pull-down.

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  Immunoprecipitation (IP) of GFP with Spot-Trap Magnetic Particles M-270: Coomassie and Western blot (I: Input, FT: Flow-Through, B: Bound).

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  Application of Spot-tag related products.

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  Cartoon to visualize the binding of large protein with Spot-tag (Spot-tag (green) + protein of interest (POI, blue) with interacting partner X (Prot X, orange) or multimeric proteins (tag (green) + protein of interest (POI, blue) to Spot-Nanobody (dark green) of Spot-Trap Magnetic Agarose or Spot-Trap Magnetic Particles M-270.

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

Cat No. etdk

Specificity

Spot-Tag^®

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic Particles M-270

Synonyms

etdk, etdk-20, Spot Trap Magnetic Particles M-270, Spot-Trap Magnetic Particles M-270, Spot-Trap Magnetic Particles M-270 Kit, Spot-Trap® Magnetic Particles M-270 Kit, etdk-20, PDRVRAVSHWSS, spot, spot tag, spot-tag, Spot-Trap® Magnetic Particles M-270

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Product Information

Spot-Trap® Magnetic Particles M-270 are affinity beads for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to Magnetic Particles M-270.

• Description: The Spot-Trap Magnetic Particles M-270 Kit contains Spot-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of Spot-tagged proteins and their interacting factors.

  • No contaminating heavy and light antibody chains

  • Extraordinary low background compared to other affinity resins

  • Recombinantly expressed without batch-to-batch variation

  • Well-characterized and validated

  • Compatible with harsh buffer conditions

  • Suitable for downstream mass spec analysis

  • Short incubation time of 30-60 minutes

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: Spot-Tag ^® sequence PDRVRAVSHWSS
• Binding capacity: 2.25 μg of recombinant Spot-tagged protein (~30 kDa) per 25 μL bead slurry
• Conjugate: Magnetic Particles M-270, size: 2.8 µm; high throughput-compatible
• Elution buffer: SDS sample buffer; 10 mM NaOH pH 12
• Wash buffer compatibility: 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 2 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 6 nM
• Compatibility with mass spectrometry: The Spot-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2861252
• Storage Buffer: PBS with 0.09% sodium azide
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
• Spot-Tag® Origin: Engineered 12 amino acid sequence modified from the unstructured N-terminus of beta-catenin
• Cross Reactivity: The Spot-Nanobody shows minimal cross reactivity with beta-catenin.

Kit components

• Component: Description
• Lysis buffer: Optimized for cytoplasmatic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Spot-Trap Magnetic Particles M-270: 20 reactions (500 µL)

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