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ChromoTek Spot-Trap® Agarose

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

Specificity

Spot-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : eta

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap

Validation Data Gallery

  • Immunoprecipitation of Spot-tagged protein.

    Immunoprecipitation of Spot-tagged protein.

  • Immunoprecipitation of Spot-tagged protein. Input (I), non-bound (FT) and bound (B), Coomassie staining and Western blotting. Western blot indicates high effectivity.

    Immunoprecipitation of Spot-tagged protein. Input (I), non-bound (FT) and bound (B), Coomassie staining and Western blotting. Western blot indicates high effectivity.

  • Elution of Spot-tag fusion protein. Input (I), non-bound (FT) and eluted (E1, E2 and E3) fractions. Elutions with Spot-peptide.

    Elution of Spot-tag fusion protein. Input (I), non-bound (FT) and eluted (E1, E2 and E3) fractions. Elutions with Spot-peptide.

  • Application of Spot-tag related products.

    Application of Spot-tag related products.

Product Information

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
ClonalityMonoclonal
HostAlpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-Tag sequence PDRVRAVSHWSS
Coupled VHHRecombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 40 µm
Binding capacity10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa)
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Biomolecules

Nanobodies Right in the Middle: Intrabodies as Toolbox to Visualize and Modulate Antigens in the Living Cell.

Authors - Teresa R Wagner
View Article
IP

J Biol Chem

Serine phosphorylation of the small phosphoprotein ICAP1 inhibits its nuclear accumulation.

Authors - Valerie L Su
View Article