ChromoTek Spot-Trap® Agarose
Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.
Ready to use reagent
Specificity
Spot-Tag
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
Cat no : eta
Synonyms
Validation Data Gallery
Product Information
Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 6 nM |
Specificity/Target | Spot-Tag sequence PDRVRAVSHWSS |
Coupled VHH | Recombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 40 µm |
Binding capacity | 10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa) |
Elution buffer | SDS sample buffer 10 mM NaOH pH 12 |
Storage Buffer | 20% ethanol |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
---|
SDS Spot-Trap Agarose (PDF) |
Application notes |
---|
Applications note Spot-System for for CRISPR’ed Spot-Tag |
Poster |
---|
Scientific Spot system poster |
Product flyer |
---|
Spot-Tag, Spot-Label & Spot-Trap (PDF) |
Protocol for immunoprecipitation |
---|
Protocol Spot-Trap Agarose (PDF) |
Troubleshooting |
---|
Troubleshooting guide immunoprecipitation (PDF) |
Publications
Application | Title |
---|---|
Biomolecules Nanobodies Right in the Middle: Intrabodies as Toolbox to Visualize and Modulate Antigens in the Living Cell. | |
J Biol Chem Serine phosphorylation of the small phosphoprotein ICAP1 inhibits its nuclear accumulation. |