ChromoTek Spot-Trap® Agarose

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

Specificity

Spot-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Publications

3

Cat no : eta

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap

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Validation Data Gallery

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  Immunoprecipitation of Spot-tagged protein.

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  Immunoprecipitation of Spot-tagged protein. Input (I), non-bound (FT) and bound (B), Coomassie staining and Western blotting. Western blot indicates high effectivity.

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  Elution of Spot-tag fusion protein. Input (I), non-bound (FT) and eluted (E1, E2 and E3) fractions. Elutions with Spot-peptide.

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  Application of Spot-tag related products.

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Product Information

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 6 nM
• Specificity/Target: Spot-Tag sequence PDRVRAVSHWSS
• Coupled VHH: Recombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 40 µm
• Binding capacity: 10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa)
• Elution buffer: SDS sample buffer; 10 mM NaOH pH 12
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	Biomolecules; Nanobodies Right in the Middle: Intrabodies as Toolbox to Visualize and Modulate Antigens in the Living Cell.; Authors - Teresa R Wagner
IP	J Biol Chem; Serine phosphorylation of the small phosphoprotein ICAP1 inhibits its nuclear accumulation.; Authors - Valerie L Su
	FEBS J; A guide to: generation and design of nanobodies.; Authors - Serge Muyldermans