ChromoTek Spot-Trap® Agarose

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

Ready to use reagent

Specificity

Spot-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Cat no : eta

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap



Product Information

Spot-Trap® Agarose is an affinity bead for immunoprecipitation (IP) of Spot-tagged proteins It comprises an anti-Spot-Tag® VHH/ Nanobody coupled to agarose beads.

ApplicationsIP, CoIP, ChIP, RIP
Clonality Monoclonal
Host Alpaca
AffinityDissociation constant KD of 6 nM
Specificity/TargetSpot-Tag sequence PDRVRAVSHWSS
Coupled VHHRecombinant, monoclonal anti-Spot-Tag single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 40 µm
Binding capacity10 µL slurry bind ~ 7 µg of recombinant Spot-Tag fusion protein (30 kDa)
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Biomolecules

Nanobodies Right in the Middle: Intrabodies as Toolbox to Visualize and Modulate Antigens in the Living Cell.

Authors - Teresa R Wagner
IP

J Biol Chem

Serine phosphorylation of the small phosphoprotein ICAP1 inhibits its nuclear accumulation.

Authors - Valerie L Su