Streptococcus Strep Tag II Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85798-1-RR (same clone as 85798-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85798-1-RR (Strep Tag II antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85798-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 85798-1-RR (same clone as 85798-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using Strep Tag II antibody (85798-1-RR, Clone: 243000G10 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green). This data was developed using the same antibody clone with 85798-1-PBS in a different storage buffer formulation.

Strep Tag II Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85798-1-PBS

Clone No.243000G10

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Strep, Strep-Tag, Strep-Tag II

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Product Information

85798-1-PBS targets Strep Tag II in WB, IF/ICC, Indirect ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Strep Tag II
• Calculated Molecular Weight: 1 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Strep Tag II
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Strep-Tag II consists of 8 amino acids with the sequence of Trp-Ser-His-Pro-Gln-Phe-Glu-Lys. Compared with Strep-Tag, Strep-Tag II has a higher binding affinity with streptavidin and can be constructed at the N-terminus or C-terminus of the target protein, providing greater flexibility.