Strep Tag II Recombinant monoclonal antibody

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  WB analysis using 85798-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85798-1-RR (Strep Tag II antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected HEK-293 using 85798-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using Strep Tag II antibody (85798-1-RR, Clone: 243000G10 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

Strep Tag II Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85798-1-RR

Clone No.243000G10

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, ELISA

Synonyms

Strep, Strep-Tag, Strep-Tag II

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Tested Applications

• Positive WB detected in: Transfected HEK-293T cells
• Positive IF/ICC detected in: Transfected HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85798-1-RR targets Strep Tag II in WB, IF/ICC, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Strep Tag II
• Calculated Molecular Weight: 1 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Strep Tag II
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Strep-Tag II consists of 8 amino acids with the sequence of Trp-Ser-His-Pro-Gln-Phe-Glu-Lys. Compared with Strep-Tag, Strep-Tag II has a higher binding affinity with streptavidin and can be constructed at the N-terminus or C-terminus of the target protein, providing greater flexibility.