Filter: AllIHCAffinity

IHC staining of mouse spleen using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat spleen using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat spleen using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 84069-8-RR
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84069-8-RR (Syndecan-1/CD138 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Affinity and Kinetic Characterization of 84069-8-RR
Biolayer interferometry (BLl) kinetic assays of 84069-8-RR against Mouse CD138/Syndecan-1 were performed. The affinity constant is 1.15 nM.

CD138/Syndecan-1 Recombinant monoclonal antibody

Name: CD138/Syndecan-1 Recombinant monoclonal antibody
Brand: Proteintech
SKU: 84069-8-RR

CD138/Syndecan-1 Uni-rAb^® Recombinant Antibody for IHC, ELISA

Cat No. 84069-8-RR

Clone No.241278E8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Synonyms

CD138, Sdc1, SYND1, Synd-1, Syndecan-1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in

mouse spleen tissue, human tonsillitis tissue, mouse colon tissue, mouse skin tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84069-8-RR targets CD138/Syndecan-1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1221 Product name: Recombinant Mouse CD138/Syndecan-1 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 23-252 aa of NM_011519.2 Sequence: QIVAVNVPPEDQDGSGDDSDNFSGSGTGALPDTLSRQTPSTWKDVWLLTATPTAPEPTSSNTETAFTSVLPAGEKPEEGEPVLHVEAEPGFTARDKEKEVTTRPRETVQLPITQRASTVRVTTAQAAVTSHPHGGMQPGLHETSAPTAPGQPDHQPPRVEGGGTSVIKEVVEDGTANQLPAGEGSGEQDFTFETSGENTAVAAVEPGLRNQPPVDEGATGASQSLLDRKE Predict reactive species
Full Name	syndecan 1
Calculated Molecular Weight	33 kDa
GenBank Accession Number	NM_011519.2
Gene Symbol	Sdc1
Gene ID (NCBI)	20969
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P18828-1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.