TAB1 Monoclonal antibody

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  WB analysis of HEK-293 using 67020-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67020-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67020-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67020-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67020-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67020-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 67020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 67020-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TAB1 antibody (67020-1-Ig, Clone: 1E10D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

TAB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67020-1-Ig

Clone No.1E10D12

Publications(2)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

MAP3K7IP1, Mitogen-activated protein kinase kinase kinase 7-interacting protein 1, TAK1 binding protein 1, TAK1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HSC-T6 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

67020-1-Ig targets TAB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6590

  Product name: Recombinant human MAP3K7IP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-367 aa of BC050554

  Sequence: MAAQRRSLLQSEQQPSWTDDLPLCHLSGVGSASNRSYSADGKGTESHPPEDSWLKFRSENNCFLYGVFNGYDGNRVTNFVAQRLSAELLLGQLNAEHAEADVRRVLLQAFDVVERSFLESIDDALAEKASLQSQLPEGVPQHQLPPQYQKILERLKTLEREISGGAMAVVAVLLNNKLYVANVGTNRALLCKSTVDGLQVTQLNVDHTTENEDELFRLSQLGLDAGKIKQVGIICGQESTRRIGDYKVKYGYTDIDLLSAAKSKPIIAEPEIHGAQPLDGVTGFLVLMSEGLYKALEAAHGPGQANQEIAAMIDTEFAKQTSLDAVAQAVVDRVKRIHSDTFASGGERARFCPRHEDMTLLVRNFGY

• Full Name: mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC050554
• Gene Symbol: TAB1
• Gene ID (NCBI): 10454
• RRID: AB_2882336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Hum Mutat; The MAP3K7 gene: further delineation of clinical characteristics and genotype/phenotype correlations.; Authors - Geeske M van Woerden
human	WB,IP	Leukemia; Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15; Authors - Cong Liu