TAB1 Monoclonal antibody

TAB1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1E10D12

Cat no : 67020-1-Ig

Synonyms

3' Tab1, MAP3K7IP1, TAB1, TAK1 binding protein 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 67020-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67020-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67020-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67020-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67020-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67020-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67020-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 67020-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TAB1 antibody (67020-1-Ig, Clone: 1E10D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, MCF-7 cells, HSC-T6 cells, K-562 cells, HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

67020-1-Ig targets TAB1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: TAB1 fusion protein Ag6590
• Full Name: mitogen-activated protein kinase kinase kinase 7 interacting protein 1
• Calculated molecular weight: 55 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC050554
• Gene symbol: MAP3K7IP1
• Gene ID (NCBI): 10454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Hum Mutat; The MAP3K7 gene: further delineation of clinical characteristics and genotype/phenotype correlations.; Authors - Geeske M van Woerden